Reproduction Abstracts

Neurons essential for reproductive functions are distributed in hypothalamus. The precise functions of these neurons are not fully analyzed at a cellular level, especially in domestic animals. To analyze cellular mechanisms that control ruminant reproduction, we generated neuronal cell lines by immortalizing fetal goat hypothalamus.The hypothalamic arcuate nucleus was collected from a female Shiba goat at E118. The tissue was dispersed and plated. Twenty...

Introduction: Pulsatile GnRH secretion is a key determinant for normal gonadal activities. Recent emerging evidence suggests that neurokinin B (NKB) plays a pivotal role in generating GnRH pulse. Here, we aimed to develop novel compounds with potent NKB receptor (NK3R) agonistic activity for future therapeutic applications to improve gonadal functions.Materials and methods: On the basis of the structure-activity relationship study of NKB and tachykinin p...

The aim of the present study was to evaluate ovarian and hormonal responses to the administration of metastin/kisspeptin analog, TAK-683, under the endocrine environments of luteal and follicular phases in goats. At 7–10 days after the confirmation of ovulation by ultrasonography, six goats received a prostaglandin F2α (PGF2α) injection (Day 0) followed by 10 days of CIDR-G® treatment. PGF2α was injected agai...

The aim of the present study was to evaluate ovarian and hormonal responses to follicular phase administration of a metastin/kisspeptin analog, TAK-683, with a low dose, determined as the minimally effective dose for LH stimulation in ovariectomized goats. At 7–10 days after ovulation, goats received a prostaglandin F2α (PGF2α) injection (day 0) followed by 10 days of CIDR-G® treatment. A PGF2α was injected a...